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  1. Compute maximum reaction rate and rate constant (1/[S] vs [S]/V plot)./Compute maximum reaction rate and rate constant ([S] vs [S]/V plot)./Compute maximum reaction rate and rate constant (V/[S] vs. V plot).
  2. Compute molecular weight using sedimentation equilibrium data.
  3. Compute molecular weight using velocity-diffusion data
  4. Compute the pH for (HA + OH- = A- + H2O) buffer system using Ka/Compute the pH for (HA + OH- = A- + H2O) buffer system using pKa
  5. Estimate molecular weight of a macromolecule using sedimentation constants.


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